Fig. 2: The lncRNA GAS5 negatively regulates the adipogenic differentiation of MSCs. | Cell Death & Disease

Fig. 2: The lncRNA GAS5 negatively regulates the adipogenic differentiation of MSCs.

From: The long noncoding RNA GAS5 negatively regulates the adipogenic differentiation of MSCs by modulating the miR-18a/CTGF axis as a ceRNA

Fig. 2

a, b ORO and quantification in GAS5-overexpressing cells. c Expression of mRNAs encoding the adipogenic markers PPAR-γ, C/EBP-α, and FABP4 in MSCs overexpressing GAS5. d Protein levels of the adipogenic markers PPAR-γ, C/EBP-α, and FABP4 in MSCs overexpressing GAS5. e Cell cycle analysis of MSCs overexpressing GAS5. f, g ORO staining and quantification in GAS5-knockdown cells. h Expression of mRNAs encoding the adipogenic markers PPAR-γ, C/EBP-α, and FABP4 in GAS5-knockdown MSCs. i Protein levels of the adipogenic markers PPAR-γ, C/EBP-α, and FABP4 in GAS5-knockdown MSCs. j Cell cycle analysis of GAS5-knockdown MSCs through PI staining. The results are presented as the means ± SD (*P < 0.05, **P < 0.01, as determined by Student’s t-test, n = 3). All experiments were performed three independent times

Back to article page