Fig. 1: Global profiling of microRNA expression in tissues from PDAC mouse models. | Cell Death & Disease

Fig. 1: Global profiling of microRNA expression in tissues from PDAC mouse models.

From: MiR-142-3p is downregulated in aggressive p53 mutant mouse models of pancreatic ductal adenocarcinoma by hypermethylation of its locus

Fig. 1

a Agilent microRNA microarrays were used to investigate global microRNA expression profiles from Kras Ptenflox (n = 4), Kras p53flox (n = 5) and Kras p53R172H (n = 5)-expressing mouse pancreatic tumour tissues. Heat map shows the hierarchal clustering and expression levels of microRNAs for all the samples in the analysis. Statistical analysis and fold changes presented in Table 1 were calculated using a one-way ANOVA with post hoc analysis using Tukey’s honest significance difference test (HSD) with an FDR (Benjamini Hochberg)-adjusted p value cutoff of 0.05. A list of all statistically significantly changed microRNAs can be found in supplemental Fig. 1. b Venn diagram demonstrating the relationship between miRNAs differentially expressed in Kras p53R172H/+ vs Kras Ptenflox and Kras p53R172H/+ vs Kras p53flox tumours. c RT-qPCR was used to validate the microarray data using the ΔΔCT method with U6 snRNA as the reference gene. A two-sample, two-tailed, unpaired t-test was used to compare the ΔCT values from each group. Statistical significance is represented as *p < 0.05, **p < 0.01 and ***p < 0.005

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