Fig. 6: Bisulphite sequencing reveals increased methylation around the miR-142 genomic locus in the Kras p53R172H cell line.

a 378 bp fragment of a CpG island which contains 18 CpGs and overlaps the miR-142 genomic locus was amplified and investigated using bisulphite sequencing. A schematic shows the location of the CpG island. The lollipop plot shows the location of the CpGs with black spots representing methylated CpGs and white spots representing non-methylated CpGs (a). A two-tailed Mann–Whitney U-test was used to assess for statistically significant differences between the two bisulphite sequencing groups