Fig. 2: Follicular morphology.
From: Loss of PUMA protects the ovarian reserve during DNA-damaging chemotherapy and preserves fertility
a Representative images of PAS-stained ovarian cortex from mice of the indicated genotypes harvested 5 days following treatment with saline, cyclophosphamide or cisplatin. Dashed inset boxes denote areas shown in higher magnification to the right. Black arrowheads denote primordial follicles. White arrowheads denote residual follicular structures without a surviving central oocyte. Scale bar = 20 µm. b Representative images of healthy pre-antral (i), healthy antral (ii), atretic pre-antral (iii) and atretic antral follicles (iv). Scale bars = 50 µm. c Quantification of atretic follicles (per animal; n = 4–5). Data expressed as mean + SEM. d Representative images of longitudinal cross-sections through ovaries of WT, Puma−/− and TAp63−/− female mice that had been treated with saline, cyclophosphamide or cisplatin. Scale bars = 200 µm e. Ovarian volume (µm3/ovary) of mice of the indicated genotypes 5 days following treatment (n = 4–5). Data are expressed as mean + SEM *p ≤ 0.05 compared with WT control (one-way ANOVA, Tukey’s multiple comparisons test)
