Fig. 3: Generation of G6PD overexpressing cell lines. Differences with mock transfection after polydatin treatment.

a Viability assay (MTT) in G6PD+ and mock-transfected cells following polydatin treatment. b Quantitative real time PCR on G6PD+ and mock-transfected cells for CHOP and spliced XBP1 after polydatin treatment. c IF with ER-Tracker on G6PD+ and mock-transfected cells after polydatin treatment. d Flow cytometry of CellRox (oxidative stress determination) on G6PD+ and mock-transfected cells following polydatin treatment. e Invasion assay in G6PD+ and mock-transfected cells. *p < 0.05, N = 3, error bar = 95% confidence