Fig. 4: Analysis of Mtb H3 localization within autophagosomal/autolysosomal vesicles. | Cell Death & Disease

Fig. 4: Analysis of Mtb H3 localization within autophagosomal/autolysosomal vesicles.

From: Clinical isolates of the modern Mycobacterium tuberculosis lineage 4 evade host defense in human macrophages through eluding IL-1β-induced autophagy

Fig. 4

Primary macrophages were infected with H3 and H37Rv Mtb. Cells were fixed and analyzed for LC3 (a) or LAMP1 (b) localization by immunofluorescence using specific antibodies, while Mtb was detected by auramine staining. The images show that the merging of the two fluorescence signals is shown on the left panels. Green: Mtb; red: LC3 in (a), LAMP1 in (b). Scale bar, 6 μm. Colocalization rate was measured by Mander’s coefficient calculated by ImageJ software. Graphics reporting a quantification of the experiments is shown in the right panels. The results represent the mean ± SD of three independent experiments

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