Fig. 2: Hirsutine induces mitochondrial injury in A549 cells. | Cell Death & Disease

Fig. 2: Hirsutine induces mitochondrial injury in A549 cells.

From: Hirsutine induces mPTP-dependent apoptosis through ROCK1/PTEN/PI3K/GSK3β pathway in human lung cancer cells

Fig. 2

a A549 cells were treated with 80 μM hirsutine for 24 h, mitochondria were observed by transmission electron microscopy. Scale bars: 1 μm. b A549 cells were treated with various concentrations of hirsutine for 24 h. ATP concentrations were determined using an ATP Determination Kit. c, d Mitochondrial membrane potential as analyzed by confocal microscopy or microplate reader using JC-1 staining. Scale bars: 20 μm. Ratio of red/green fluorescence intensity represents the potential of the mitochondrial membrane. e Intracellular ROS level was detected by DCF-DA staining; mean value of ROS fluorescence intensity was measured by flow cytometry. f Mitochondrial (Mito) and cytosolic (Cytosol) fractions were prepared and subjected to western blot analysis. The relative intensities of Cyto C in cytosolic fractions were normalized to Actin by densitometric analysis using Quantity One software. Data are expressed as the mean ± SD (n = 3). The results were expressed as a percentage of control, which was set at 100%, **P < 0.01 vs. the control group

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