Fig. 4: Effect of Ngb overexpression on Ngb expression and neuronal cells damage in CA1 after ischemia with or without HPC. | Cell Death & Disease

Fig. 4: Effect of Ngb overexpression on Ngb expression and neuronal cells damage in CA1 after ischemia with or without HPC.

From: Neuroglobin mediates neuroprotection of hypoxic postconditioning against transient global cerebral ischemia in rats through preserving the activity of Na+/K+ ATPases

Fig. 4

A Design of experiments in which rats were stereotaxically injected bilaterally with Ngb lentiviral vectors in the dorsal CA1 pyramidal layer and subjected to either Sham-operation or tGCI with or without hypoxia. B Phase contrast and fluorescent images from the coronal sections of CA1 following injection of Ngb lentiviral vectors in Sham-operated animals. C Representative photomicrographs show the co-localization of Ngb (red), GFP (green), and 4′,6-diamidino-2-phenylindole (DAPI) (blue) in the brain sections from animals at 7 days after tGCI with Lenti-Ngb injection. D Cresyl violet stained and NeuN-immunostained hippocampal sections from rats administered bilaterally with either Lenti-control or Lenti-Ngb at 7 days after reperfusion with or without hypoxia. Boxes indicate the magnified regions displayed in the right panel. E, F Quantitative analyses of the survival cells and NeuN-positive cells in the CA1. Each bar represents the mean ± S.D. *p < 0.05 vs. Sham-operated animals with injection of Lenti-control, #p < 0.05 vs. tGCI group with injection of Lenti-control (n = 6 in each group). G Representative immunoblots of Ngb expression in the CA1. The histogram presents the quantitative analyses of Ngb levels. Data are expressed as percentage of value of the Sham-operated animals. Each bar represents the mean ± S.D. *p < 0.05 vs. Sham-operated animals, #p < 0.05 vs. Sham-operated group with injection of Lenti-control, and &p < 0.05 vs. HPC group with injection of Lenti-control (n ≥ 3 in each group)

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