Fig. 5: Effect of HPC on the protein expression and cellular localization of Atp1b1 in the CA1 after tGCI. | Cell Death & Disease

Fig. 5: Effect of HPC on the protein expression and cellular localization of Atp1b1 in the CA1 after tGCI.

From: Neuroglobin mediates neuroprotection of hypoxic postconditioning against transient global cerebral ischemia in rats through preserving the activity of Na+/K+ ATPases

Fig. 5

A Representative immunoblots of Atp1b1 expression in total proteins in the CA1. The histogram presents the quantitative analyses of Atp1b1 levels (n = 3 in each group). B The immunoblot analysis of Atp1b1 protein levels in the cytoplasmic and the membrane fraction from CA1. Data are expressed as percentage of value of Sham-operated animals. Each bar represents the mean ± S.D. *p < 0.05 vs. Sham-operated animals, #p < 0.05 vs. tGCI group (n ≥ 3 in each group). C Immunohistochemistry of Atp1b1 in the rat brains. Representative images show the Sham-operated group (a and b), 168 h after reperfusion of the tGCI groups (c and d), and 168 h after reperfusion of the HPC groups (e and f), respectively. Boxes indicate the magnified regions displayed in the right panel. D Quantitative analysis of Atp1b1-positive cells in the CA1. Each bar represents the mean ± S.D. *p < 0.05 vs. Sham-operated animals and #p < 0.05 vs. tGCI group (n = 6). E Representative photomicrographs of fluorescent double staining show that Atp1b1 (green) was mainly colocalized with GFAP (red) (d-f) rather than NeuN (red) (a-c) in the CA1 at 168 h after tGCI. However, Atp1b1 colocalized with NeuN (g-i), but not GFAP (red) (j-l) at 168 h after tGCI with hypoxia. Scale bar: 50 μm

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