Fig. 6: The regulation of Ngb to membrane protein of Atp1b1 in the CA1 after tGCI with or without HPC. | Cell Death & Disease

Fig. 6: The regulation of Ngb to membrane protein of Atp1b1 in the CA1 after tGCI with or without HPC.

From: Neuroglobin mediates neuroprotection of hypoxic postconditioning against transient global cerebral ischemia in rats through preserving the activity of Na+/K+ ATPases

Fig. 6

A Immunoprecipitation blots showing the Ngb–Atp1b1 complex in the CA1 of the tGCI and the HPC groups. Atp1b1 was immunoprecipitated (IP) using anti-Atp1b1 antibody. Ngb was detected by western blot (WB). The experiments were repeated twice (n = 3 in each group). B Representative photomicrographs of proximity ligation assay showed the interaction between Ngb and Atp1b1 in the Sham-operated group (a), 26 h after reperfusion of the tGCI groups (b), and 26 h after reperfusion of the HPC groups (c). Dots (red) indicate Ngb–Atp1b1 complexes. Scale bar: 25 μm. C Quantitative analysis of PLA signal in CA1. Each bar represents the mean ± S.D. *p < 0.05 vs. Sham-operated animals and #p < 0.05 vs. tGCI group at the same time point. D Immunoblot analysis of Atp1b1 in the cytoplasmic and the membrane fraction from CA1 of Sham and HPC groups with injection of Ngb ODNs. The values are expressed as percentage of value of Sham-operated animals. *p < 0.05 vs. Sham-operated animals and #p < 0.05 vs. HPC group with S-ODNs administration (n ≥ 3 in each group). E Immunoblot analysis of Atp1b1 in the cytoplasmic and the membrane fraction from Sham-operated and tGCI group with injection of Lenti-control or Lenti-Ngb. Data are expressed as percentage of the value of Sham-operated animals. *p < 0.05 vs. Sham-operated animals, #p < 0.05 vs. Sham-operated group with injection of Lenti-control, and &p < 0.05 vs. HPC group with injection of Lenti-control (n ≥ 3 in each group)

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