Fig. 2: OX40 controls cDNT survival by its regulation on Survivin, Bcl-2, Bcl-xL, and BCL2L11.
From: Critical role of OX40 in the expansion and survival of CD4 T-cell-derived double-negative T cells
a Flow cytometric analysis of Bcl-2, Bcl-xl, and Survivin protein expression in B6 cDNT and OX40 KO cDNT after anti-CD3/CD28 stimulation for 48 h. b Relative mRNA expression of anti-apoptosis genes (Bcl-2, Bcl-xl, and Survivin) and pro-apoptosis genes (Bcl2l11) in B6 cDNT and OX40 KO cDNT after incubation with CD3/CD28 for 48 and 72 h. c The relative mRNA levels of NF-κB signaling genes (nfkb1 and rela) in OX40 KO cDNT compared with WT cDNT. d Flow cytometric analysis of RelA, and phosphorylation of IκBα and IKKα/β protein expression in B6 cDNT and OX40 KO cDNT cells after anti-CD3/CD28 stimulation for 48 h. e Nuclear protein extracts were prepared from B6 cDNT cells and OX40 KO cDNT cells after anti-CD3/CD28 stimulation for 48 h. The RelA and p50 protein levels in the cells were analyzed by western blot. The relative density of the RelA and p50 band was normalized to Histone H3. The data are presented as the mean ± SD, n = 5 in each group. *p < 0.05. NS no significance
