Fig. 6: IL-2 regulated OX40 expression through transcription factor PPARα.
From: Critical role of OX40 in the expansion and survival of CD4 T-cell-derived double-negative T cells
a Inhibition of OX40 mRNA levels after incubation with or without IL-2 for 48 h by a PPARα agonist (80 µM). b The percentage of OX40+ cDNT was detected after stimulation with a PPARα agonist in the presence or absence of IL-2. Statistical analysis of OX40+ cells proportion stimulation with IL-2 relative to the proportion without IL-2 in each group, as determined by flow cytometry. c Relative mRNA levels of anti-apoptosis genes (Bcl-2, Bcl-xl, and Survivin) and pro-apoptosis genes (Bcl2l11) of cDNT after incubation with a PPARα agonist with or without IL-2. Statistical analysis of the genes expression stimulation with IL-2 relative to the genes without IL-2 in each group was determined. d Apoptosis of cDNT (Annexin V+) was detected after stimulation with a PPARα agonist with or without IL-2 by flow cytometry. e The proliferation of cDNT (EdU+) was detected after stimulation with a PPARα agonist with or without IL-2 by flow cytometry. The data are presented as the mean ± SD, n = 5 in each group. *p < 0.05, **p < 0.01, NS no significance
