Fig. 3: Effects of prenatal ethanol exposure (PEE, 4  g/kg per day) on hippocampal glucocorticoid receptor (GR) and glutamic acid decarboxylase 67 (GAD67) expression and the total methylation rate of the GAD67 promoter (-1019 to -691 bp) without and after chronic stress in male adult offspring rats. | Cell Death & Disease

Fig. 3: Effects of prenatal ethanol exposure (PEE, 4  g/kg per day) on hippocampal glucocorticoid receptor (GR) and glutamic acid decarboxylase 67 (GAD67) expression and the total methylation rate of the GAD67 promoter (-1019 to -691 bp) without and after chronic stress in male adult offspring rats.

From: Programming for increased expression of hippocampal GAD67 mediated the hypersensitivity of the hypothalamic–pituitary–adrenal axis in male offspring rats with prenatal ethanol exposure

Fig. 3

a, b Hippocampal GR and GAD67 mRNA expression. c Photomicrographs of immunohistochemistry for hippocampal GAD67; the brown signal was distributed in the cytoplasm and vesicles of hippocampal neurons. d, e Quantitative analysis of GAD67 in the hippocampal CA3 and DG areas; three brain sections from different levels of the hippocampus were selected from each animal and quantified. f Total methylation rate of the GAD67 promoter (-1019 to -691 bp); % methylation was used to quantify the methylations status of the GAD67 promoter (-1019 to -691 bp). Mean ± S.E.M., n = 8 offspring from eight litters. *P < 0.05, **P < 0.01 vs. control

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