Fig. 7: Effects of corticosterone (20–500  ng/ml) and mifepristone (RU486, 5  μM) on glutamatergic (glutamate)/GABAergic (GAD67) neuron numbers, the expression of glucocorticoid receptor (GR), glutamic acid decarboxylase 67 (GAD67) and DNA methylation transferase 1 (DNMT1) and the total methylation rate of the GAD67 promoter region (-1019 to -691 bp) in the foetal hippocampal cell line H19-7. | Cell Death & Disease

Fig. 7: Effects of corticosterone (20–500  ng/ml) and mifepristone (RU486, 5  μM) on glutamatergic (glutamate)/GABAergic (GAD67) neuron numbers, the expression of glucocorticoid receptor (GR), glutamic acid decarboxylase 67 (GAD67) and DNA methylation transferase 1 (DNMT1) and the total methylation rate of the GAD67 promoter region (-1019 to -691 bp) in the foetal hippocampal cell line H19-7.

From: Programming for increased expression of hippocampal GAD67 mediated the hypersensitivity of the hypothalamic–pituitary–adrenal axis in male offspring rats with prenatal ethanol exposure

Fig. 7

a, b Photomicrographs and quantitative analysis of immunofluorescence for glutamatergic neurons (glutamate, red), GABAergic neurons (GAD67, red) and neurons (NeuN, green) (Scale bars = 200 μm, n = 6). Quantitative analysis of glutamatergic and GABAergic neurons. c, e–g The mRNA expression of GAD67, GR and DNMT1 (n = 3). D Total methylation rate of the GAD67 promoter region (-1019 to -691 bp); % methylation was used to quantify the methylations status of the GAD67 promoter (-1019 to -691 bp). Mean ± S.E.M., n = 3. *P < 0.05, **P < 0.01 vs. control

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