Fig. 3: IL-37d ameliorated LPS-induced endotoxemia in vivo.
From: IL-37 isoform D downregulates pro-inflammatory cytokines expression in a Smad3-dependent manner
a LPS (30 mg/kg) were intraperitoneally injected to IL-37dtg mice (n = 5) and WT mice (n = 4) and survival rate was analyzed. b–e LPS (10 mg/kg) were injected intraperitoneally to IL-37dtg mice (n = 13) and WT mice (n = 6) and then body temperature was measured at the indicated time points (b). The levels of IL-6, TNF-α, MCP-1, IL-1β, IFN-γ, and IL-17A in spleen were detected by qRT-PCR (c). The expressions of IL-6, TNF, MCP-1, IL-1β, IFN-γ, and IL-17A in lysates of spleen were measured by CBA. Results are presented relative to total protein (TP) (d). The levels of IL-6, TNF-α, MCP-1, IL-1β, IFN-γ, and IL-17A in serum were measured by CBA (e). *P < 0.05; **P < 0.01; ***P < 0.001, ns, no significant difference. Data are shown as the mean ± SEM
