Fig. 5: IL-37d interacted with Smad3 and promoted Smad3 nuclear translocation.
From: IL-37 isoform D downregulates pro-inflammatory cytokines expression in a Smad3-dependent manner
a The recombinant plasmids containing IL-37d-Flag and Myc-Smad3 were co-transfected into HEK-293T cells and immunoprecipitation of IL-37d with Smad3 in cell extracts was performed using anti-Flag antibody and then followed by SDS-PAGE and immunoblotting with anti-Smad3 antibody. b A549 cells were infected with IL-37d-Flag recombinant or negative control lentivirus and then stimulated with or without IL-1β (10 ng/ml) for 0.5 h. Co-localization of IL-37d (Alexa-594, green) with phosphor-Smad3 (Alexa-647, red) and nuclei (DAPI, blue) was observed. Scale bar, 10 μm. c A549 cells expressing IL-37d-Flag were treated with or without IL-1β (10 ng/ml) for 0.5 h and then the expressions of pSmad3 and IL-37d in the nuclear and cytoplasmic extracts were detected by western blot. Data are representative of three independent experiments
