Fig. 4: The combination of vorinostat and QC blocks mitophagy and accumulation of mito-aggresomes. | Cell Death & Disease

Fig. 4: The combination of vorinostat and QC blocks mitophagy and accumulation of mito-aggresomes.

From: Vorinostat and quinacrine have synergistic effects in T-cell acute lymphoblastic leukemia through reactive oxygen species increase and mitophagy inhibition

Fig. 4

(a, b, d) Jurkat cells were treated with vorinostat (1 μM) and/or QC (5 μM) for 36 h. a The indicated proteins in the cytoplasm and mitochondria were examined by western blot. b TEM of cells; the red arrows indicate the damaged mitochondria that are wrapping in double-membrane structures. c Jurkat cells treated with vorinostat (1 μM) and/or QC (5 μM) in the presence or absence of NAC (10 mM) for 36 h, and then the indicated proteins were examined by western blot. d The expression levels of K63-linked and K48-linked ubiquitinated proteins in the mitochondria were examined by western blot. All experiments were performed at least three times with the same results

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