Fig. 1: MC-3129 induces apoptosis in multiple leukemia cell lines. | Cell Death & Disease

Fig. 1: MC-3129 induces apoptosis in multiple leukemia cell lines.

From: The cyclohexene derivative MC-3129 exhibits antileukemic activity via RhoA/ROCK1/PTEN/PI3K/Akt pathway-mediated mitochondrial translocation of cofilin

Fig. 1

U937 cells were treated with various MC-3129 concentrations for 24 h or with 10 μM of MC-3129 for different time intervals, as indicated. a, b The cell apoptosis was determined by flow cytometry using Annexin V/PI staining, the percentage of apoptotic cells was analyzed for three separate experiments (mean ± SD, ns P > 0.05, **P < 0.01 compared with control). c, d Whole cell lysates, cytosolic (Cytosol) and mitochondrial (Mito) fractions from U937 cells were prepared and subjected to immunoblotting using antibodies against PARP, cleaved-caspase-3 (C-Caspase-3), cleaved-caspase-9 (C-Caspase 9), cytochrome c (Cyto c), GADPH, and Cox IV. Jurkat, HL-60, and K562 cells were treated with or without 10 μM of MC-3129 for 24 h. e The cell apoptosis was determined by flow cytometry, values represent the means ± SD, for three separate experiments (**P < 0.01). f The total protein lysates were analyzed by immunoblotting using the indicated antibodies. CF cleavage fragment

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