Fig. 4: DRP1 contributes to ER stress-induced inflammasome activation. | Cell Death & Disease

Fig. 4: DRP1 contributes to ER stress-induced inflammasome activation.

From: The kinase receptor-interacting protein 1 is required for inflammasome activation induced by endoplasmic reticulum stress

Fig. 4

a The expression level of DRP1 in J774A.1 macrophages transfected with control siRNA with a scrambled sequence (Scr) or DRP1-specific siRNA. b, c Release of IL-1β and TNF-α by J774A.1 macrophages which were first transfected with control siRNA (Scr) or DRP1-specific siRNA and then treated with LPS plus TG or LPS plus Nigericin. d Immunoblot analysis of cleaved caspase-1 (p20) in cell supernatants (SN) and caspase-1 (pro-casp1) and β-actin in cell lysates (Input) of J774A.1 macrophages which were first transfected with control siRNA (Scr) or DRP1-specific siRNA and then treated with LPS plus TG or LPS plus Nigericin. e, f Secretion of IL-1β and TNF-α by BMDMs stimulated by LPS plus TG or LPS plus Nigericin with or without DRP1 inhibitor Mdivi-1 pretreatment. g Immunoblot of cleaved caspase-1 (p20) in cell supernatants (SN) and caspase-1 (pro-casp1) and β-actin in cell lysates (Input) of BMDMs stimulated with LPS plus TG or LPS plus Nigericin with or without Mdivi-1 pretreatment. UT, unstimulated. h Confocal microscopy analysis of BMDMs with or without Nec-1 pretreatment and then stimulated with LPS plus TG. Cells were stained with MitoTracker. Scale bar, 25 μm. Figures are representative of at least three independent experiments. Bars indicate means plus SD. ns not significant, **P < 0.01, *P < 0.05 (unpaired Student’s t test)

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