Fig. 5: ROS is involved in inflammasome activation induced by ER stress. | Cell Death & Disease

Fig. 5: ROS is involved in inflammasome activation induced by ER stress.

From: The kinase receptor-interacting protein 1 is required for inflammasome activation induced by endoplasmic reticulum stress

Fig. 5

a ROS production detected by flow cytometry in LPS, TG, or LPS plus TG-treated BMDMs (UT unstimulated). b ROS production in LPS plus TG-treated BMDMs with or without Nec-1 pretreatment. c ROS production in LPS plus TG-treated BMDMs transfected with control siRNA (Scr) or RIP1-specific siRNAs. d ROS production in LPS plus TG-treated BMDMs with or without Midivi-1 or BHA pretreatment. e, f Secretion of IL-1β and TNF-α by BMDMs stimulated by LPS plus TG or LPS plus Nigericin with or without antioxidant BHA pretreatment. g Immunoblot of cleaved caspase-1 (p20) in cell supernatants (SN) and caspase-1 (pro caspase-1) and β-actin in cell lysates (Input) of BMDMs stimulated with LPS plus TG or LPS plus Nigericin with or without BHA pretreatment. Figures are representative of at least three independent experiments. Bars indicate means plus SD. ns not significant, **P < 0.01, *P < 0.05 (unpaired Student’s t test)

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