Fig. 4: COMP deficiency leads to mitochondrial dysfunction. | Cell Death & Disease

Fig. 4: COMP deficiency leads to mitochondrial dysfunction.

From: COMP-prohibitin 2 interaction maintains mitochondrial homeostasis and controls smooth muscle cell identity

Fig. 4

a JC-1 staining showing diminished membrane potential as confirmed by confocal fluorescence microscopy (left, Scale bar = 50 μm) and flow cytometry (right), the data were analyzed using paired two-tailed Student’s t test and presented as the means ± SD of three independent experiments. *P < 0.05. b Flow cytometry analysis of JC-1 staining in scrambled siRNA-transfected VSMCs transplanted with scrambled or COMP siRNA-transfected mitochondria. The data were analyzed using paired two-tailed Student’s t test and presented as the means ± SD of three independent experiments. *P < 0.05. c The mtDNA content was quantified by qPCR. The data were analyzed using unpaired two-tailed Student’s t test and presented as the means ± SD of three independent experiments. *P < 0.05. d Mitochondrial morphology was indicated in a rat VSMC using Mito-Tracker Green (left, scale bar = 5 μm). Mitochondrial length was measured using Image pro plus 6.0 software (Media Cybernetics, MD, USA), the data were analyzed using unpaired two-tailed Student’s t test and presented as the means ± SD of four independent experiments. *P < 0.05

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