Fig. 3: Knockdown of ALG3 attenuated MDR of AML cell lines. | Cell Death & Disease

Fig. 3: Knockdown of ALG3 attenuated MDR of AML cell lines.

From: RETRACTED ARTICLE: Aberrant mannosylation profile and FTX/miR-342/ALG3-axis contribute to development of drug resistance in acute myeloid leukemia

Fig. 3

a ALG3 expression was detected by qRT-PCR and western blot in AML cell lines transfected with ALG3 shRNA. b FCM was used to show the mannose levels by FITC-conjugated MAN-M and FITC-ConA on the cell surface of transfected AML cell lines.c The chemoresistance to ADR, VCR and Paclitaxel was detected in AML cell lines by CCK8 assays. d The IC50 values was calculated and presented. e The proliferative formation in response to different drugs of transfected AML cell lines were examined by colony-forming unit assay. f FCM showed the apoptosis of transfected AML cell lines in response to ADR, VCR and paclitaxel. g The key apoptosis related molecules were determined by western blot. h The tumor tissues of nude mice were presented and the volume was calculated on the 7, 14, 21, and 28 days. i Different tumor tissues were sectioned and stained with ALG3 and Ki67 by IHC staining. Data were the means±SD of triplicate determinants (*p < 0.05)

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