Fig. 4: Upregulation of ALG3 promoted MDR of AML cells. | Cell Death & Disease

Fig. 4: Upregulation of ALG3 promoted MDR of AML cells.

From: RETRACTED ARTICLE: Aberrant mannosylation profile and FTX/miR-342/ALG3-axis contribute to development of drug resistance in acute myeloid leukemia

Fig. 4

a qRT-PCR and western blot were carried out to detect ALG3 expression of different treated AML cell lines. b The expression of FITC-MAN-M and FITC-ConA was determined by FCM.c The alteration of chemosensitivity to ADR, VCR and Paclitaxel was detected in AML cell lines by CCK8 assays. d The IC50 values were subsequently calculated. e Proliferation of treated AML cells was observed by colony formation assay with the mediation of methylcellulose. f The apoptosis rate was clarified with ADR, VCR and paclitaxel treatment by FCM. g Caspase3, cleaved caspase3, PARP and cleaved PARP levels were analyzed by western blot. h The tumor tissues of nude mice was recorded and shown. i ALG3 and Ki67 expression was observed by IHC staining. Data were the means±SD of triplicate determinants (*p < 0.05)

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