Fig. 3: Expression of PKM2 in the developmental retina.
From: Pyruvate kinase M2 isoform deletion in cone photoreceptors results in age-related cone degeneration
Developmental scheme of cone and rod photoreceptors showing that cone development precedes rod development (a). After euthanization, whole P0 mouse heads were harvested, cut in half with one eye on each side, and were subjected to prefer-fixation followed by paraffin embedding. Genotyping was performed on mouse tail DNA to distinguish cone-cre PKM2-KO mice from PKM2-WT mice, as described in Methods. Sections were prepared from PKM2-WT (b, d) and cone-cre-PKM2-KO (c, e) mice and stained with hematoxylin and eosin. Panels (b) and (c) show the cross-section of the eye. Panels (d) and (e) show the cross-section of the retina. Immunohistochemistry was performed on PKM2-WT (f) and cone-cre PKM2-KO (g) retina sections with PNA (red) and PKM2 (green). Panels (h) and (i) represent the omission of primary antibodies. ONBL outer neuroblastic layer, INBL inner neuroblastic layer. Scale bar = 50 μm
