Table 1 Real-time PCR primers used to measure gene expression
From: Pyruvate kinase M2 isoform deletion in cone photoreceptors results in age-related cone degeneration
Gene | Forward primer | Reverse primer |
|---|---|---|
HIF-1α | GATGACGGCGACATGGTTTAC | CTCACTGGGCCATTTCTGTGT |
GLUT1 | TCAACACGGCCTTCACTG | CACGATGCTCAGATAGGACATC |
HK-2 | GGAACCCAGCTGTTTGACCA | CAGGGGAACGAGAAGGTGAAA |
6PGD | AGACAGGCAGCCACTGAGTT | AAGTTCTGGGTTTCGCTCAA |
G6PD | CCTACCATCTGGTGGCTGTT | TGGCTTTAAAGAAGGGCTCA |
PKM2 | ATTGCCCGAGAGGCAGAGGC | ATCAAGGTACAGGCACTACACGCAT |
ME1 | AGAGGTGTTTGCCCATGAAC | GCTGGTCGGATTACTCAAAGC |
ACC1 | GGACAGACTGATCGCAGAGAAAG | GCTGTTCCTCAGGCTCACAT |
C/EBPβ | GCAAGAGCCGCGACAAG | GGCTCGGGCAGCTGCTT |
FAS | GCTGCGGAAACTTCAGGAAAT | AGAGACGTGTCACTCCTGGACTT |
PGC1α | GCGCCGTGTGATTTAGCTT | AAAACTCAAAGCGGTCTCTCAA |
SREBP-1c | CTGGATTTGGCCCGGGGAGATTC | TGGAGCAGGTGGCGATGAGGTTC |
SWL | TGTACATGGTCAACAATCGGA | ACACCATCTCCAGAATGCAAG |
MWL | CTCTGCTACCTCCAAGTGTGG | AAGTATAGGGTCCCAGCAGA |
RHODOPSIN | CAAGAATCCACTGGGAGATGA | GTGTGTGGGGACAGGAGACT |
PDE6C | ACTCCCGAAACTTCAAGTGG | TGGGTGTGATCTCTCCATGA |
Cone-Trα | GCATCAGTGCTGAGGACAAA | CTAGGCACTCTTCGGGTGAG |
SLC25A33 | ATGCAGCTAGAACGCAAGGT | AGCTCCATCTGTGGAGGAGA |
18S | TTTGTTGGTTTTCGGAACTGA | CGTTTATGGTCGGAACTACGA |
