Fig. 6: Re-expression of Sox9 reverses miR-133b-dependent phenotypes in vitro.
From: MiR-133b targets Sox9 to control pathogenesis and metastasis of breast cancer
a Expression of Sox9 in MDA-MB-231-luc cells infected with pMSCV-Sox9 or control vector after transfection of NC or miR-133b mimics. b In vitro proliferation curves of the indicated MDA-MB-231-luc cells transfected with NC or miR-133b mimics. Representative morphology (scale bars, 100 μm) were shown in the right panel. c Colony formation assays for the indicated MDA-MB-231-luc cells after transfection of NC or miR-133b mimics. Left panel: Representative images are presented. Right panel: The number of clones were counted for each well of six plates and shown in the y axis. d Detection of Sox9 expression by western blot in MDA-MB-231-luc or BT549 cells co-transfected with NC or miR-133b mimics together with either pMSCV-Vector or pMSCV-Sox9, respectively. e Invasion assays of MDA-MB-231-luc or BT549 cells co-transfected with NC or miR-133b mimics together with either pMSCV-Sox9 or control vector, respectively. Data are presented as mean ± S.D. The symbols * denotes statistical difference (p < 0.05), whereas ** and *** represent great significant difference (p < 0.01 and p < 0.001) by a two-tailed Student’s t-test
