Fig. 1: 6-OHDA reduced the stability of Drosha in a mouse model of PD.
From: Loss of Drosha underlies dopaminergic neuron toxicity in models of Parkinson’s disease
a High panels: Drosha levels and TH-positive DA neurons in SNc of saline control mice and 6-OHDA lesioned PD mice. Saline or 0.3 ul 6-OHDA (20 μM) was injected into the SNc of mouse brain. Five days after injection, the brains were perfused with 0.9% NaCl solution and cold 4% paraformaldehyde in phosphate buffer. Then the brains were removed for immunofluorescence. The dilution ratio of Drosha was 1:100 and TH was 1:1000 (n = 3). Lower panels: The position of SNc in the midbrain. b The quantitative value of Drosha. (ANOVA test followed by Tukey HSD, *P < 0.05, ***P < 0.001, n = 3). c The number of TH-positive neurons. (ANOVA test followed by Tukey HSD, ***P < 0.001, n = 3). d Western blot analysis of Drosha level in different brain regions. Five days after injection, the brains were perfused with 0.9% NaCl solution and removed for immunoblot. An anti-Drosha antibody was used to determine the level of Drosha at a dilution ratio of 1:500. An anti-β-actin antibody was used as a loading control. The data are expressed as mean ± S.E.M. (Student’s t-test, **P < 0.01, n = 3). e Western blot analysis of p-p38 level in different brain regions. The brain was removed for immunoblot 2 days after injection. The data are expressed as mean ± S.E.M. (Student’s t-test, **P < 0.001, n = 3)
