Fig. 5: Enhancing Drosha alleviated the DA neuronal loss in 6-OHDA-induced mouse model of PD.
From: Loss of Drosha underlies dopaminergic neuron toxicity in models of Parkinson’s disease
a Drosha levels in SNc of PD mice after injection of negative control vector or adenovirus vectors expressing WT and mt5 Drosha. The adenovirus vectors were injected into the SNc of mice for 3 days before injection of 6-OHDA. Five days after 6-OHDA injection, the mouse brain was removed for immunoblot analysis. (ANOVA test followed by Tukey HSD, *P < 0.05, **P < 0.01, compared with the negetive control group. #P < 0.05, ##P < 0.01 vs. negative control vector treated with 6-OHDA. $P < 0.05 vs. WT vector treated with 6-OHDA, n = 3). b The TH-DAB immunostaining examined the dopaminergic terminal in striatum. Overexpression of Drosha correlated with the higher level of TH signal. (ANOVA test followed by Tukey HSD, ***P < 0.001, compared with the negetive control group. #P < 0.05, ###P < 0.001 vs. negative control vector treated with 6-OHDA, n = 3). c Immunocytochemical analysis of the midbrain sections of PD mice received injection of adenovirus vectors expressing WT and mt5 Drosha. Restoring the level of Drosha protected the DA neurons from the toxicity of 6-OHDA. d The quantitative value of Drosha. (ANOVA test followed by Tukey HSD, ***P < 0.001, compared with the negetive control group. ###P < 0.001 vs. negative control vector treated with 6-OHDA, n = 3). e The number of TH-positive neurons. (ANOVA test followed by Tukey HSD, ***P < 0.001, compared with the vehicle control group. #P < 0.05, ###P < 0.001 vs. control vector treated with 6-OHDA. $P < 0.05 vs. WT vector treated with 6-OHDA, n = 3)
