Fig. 1: AGEs-induced apoptosis in osteoblastic MC3T3-E1 cells. | Cell Death & Disease

Fig. 1: AGEs-induced apoptosis in osteoblastic MC3T3-E1 cells.

From: RAGE-dependent mitochondria pathway: a novel target of silibinin against apoptosis of osteoblastic cells induced by advanced glycation end products

Fig. 1

a Cell viability was determined by MTT reduction in osteoblastic cells in the presence of AGEs. Error bars indicate SEM (n = 6). b, c Flow cytometric quantification of apoptosis. Error bars indicate SEM (n = 6). d, e TUNEL staining and assay. Error bars indicate SEM (n = 300). Scale bars = 100 μm. f Representative immunoreactive bands for Bcl-2 and Bax in osteoblastic cells in the presence of AGEs. Full-length blots are presented in Supplementary Figure 4. g Quantification of immunoreactive bands for Bcl-2 and Bax relative to β-actin. Error bars indicate SEM (n = 6)

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