Fig. 2: AGEs-induced mitochondrial abnormalities in osteoblastic MC3T3-E1 cells. | Cell Death & Disease

Fig. 2: AGEs-induced mitochondrial abnormalities in osteoblastic MC3T3-E1 cells.

From: RAGE-dependent mitochondria pathway: a novel target of silibinin against apoptosis of osteoblastic cells induced by advanced glycation end products

Fig. 2

a, b Representative images showing MitoSOX staining and quantification in the indicated groups. Scale bars = 100 μm. c, d Representative images with TMRM staining and quantification in the indicated groups. Scale bars = 100 μm. e ATP production in the indicated groups. f–h Representative images of mitochondrial morphology, and measurements of mitochondrial length and density in the indicated groups. Error bars indicate SEM (n = 15) Scale bars = 10 μm. i–n Representative immunoreactive bands and quantification of immunoreactive bands for Drp1, Mfn1, Mfn2, Opa1, and Fis1 in osteoblasts in the presence of AGES. Error bars indicate SEM (n = 6). Full-length blots are presented in Supplementary Figure 4

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