Fig. 3: MitoQ, CsA, and silibinin attenuated AGEs-induced apoptosis in osteoblastic MC3T3-E1 cells. | Cell Death & Disease

Fig. 3: MitoQ, CsA, and silibinin attenuated AGEs-induced apoptosis in osteoblastic MC3T3-E1 cells.

From: RAGE-dependent mitochondria pathway: a novel target of silibinin against apoptosis of osteoblastic cells induced by advanced glycation end products

Fig. 3

a Cell viability determined by MTT reduction in osteoblastic cells in the presence of AGEs with or without MitoQ or CsA. Error bars indicate SEM (n = 6). b, c TUNEL staining and assay after treatment with MitoQ or CsA. Error bars indicate SEM (n = 300). Scale bars = 100 μm. d Representative immunoreactive bands for Bcl-2 and Bax in osteoblastic cells with (+) or without (−) treatment of MitoQ or CsA in the presence of AGEs (+) or culture medium (−). e–g Quantification of immunoreactive bands for Bcl-2 and Bax relative to β-actin. Error bars indicate SEM (n = 6). Full-length blots are presented in Supplementary Figure 4

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