Fig. 5: FPS-ZM1 attenuated AGEs-induced apoptosis in osteoblastic MC3T3-E1 cells. | Cell Death & Disease

Fig. 5: FPS-ZM1 attenuated AGEs-induced apoptosis in osteoblastic MC3T3-E1 cells.

From: RAGE-dependent mitochondria pathway: a novel target of silibinin against apoptosis of osteoblastic cells induced by advanced glycation end products

Fig. 5

a Cell viability determined by MTT reduction in osteoblastic cells in the presence of AGEs with or without FPS-ZM1. Error bars indicate SEM (n = 6). b, c TUNEL staining and assay after FPS-ZM1 treatment. Error bars indicate SEM (n = 300). Scale bars = 100 μm. d, f Representative immunoreactive bands for RAGE, Bcl-2, and Bax in osteoblast with (+) or without (−) FPS-ZM1 treatment in the presence of AGES (+) or culture medium (−). Full-length blots are presented in Supplementary Figure 4. e, g, h, i Quantification of immunoreactive bands for RAGE, Bcl-2, and Bax relative to β-actin. Error bars indicate SEM (n = 6)

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