Fig. 8: Silibinin prevented AGEs-induced osteoblastic MC3T3-E1 cells apoptosis through RAGE-dependent mitochondrial pathway. | Cell Death & Disease

Fig. 8: Silibinin prevented AGEs-induced osteoblastic MC3T3-E1 cells apoptosis through RAGE-dependent mitochondrial pathway.

From: RAGE-dependent mitochondria pathway: a novel target of silibinin against apoptosis of osteoblastic cells induced by advanced glycation end products

Fig. 8

a, b Representative images showing MitoSOX staining and quantification in the indicated groups. Scale bars = 10 μm. c, d Representative images showing TMRM staining and quantification in the indicated groups. Scale bars = 10 μm. e ATP production in the indicated groups. f–h Representative images of mitochondrial morphology, and measurements of mitochondrial length and density in the indicated groups. Error bars indicate SEM (n = 15). Scale bars = 10 μm. i Representative immunoreactive bands for Opa1 and Fis1 in osteoblastic cells in the presence of AGEs. Full-length blots are presented in Supplementary Figure 4. j, k Quantification of immunoreactive bands for Opa1 and Fis1 relative to β-actin. Error bars indicate SEM (n = 6)

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