Fig. 3: TNF-α-treated chondrocytes increases matrix degradation regulated by miR-23b-3p. | Cell Death & Disease

Fig. 3: TNF-α-treated chondrocytes increases matrix degradation regulated by miR-23b-3p.

From: Downregulation of HS6ST2 by miR-23b-3p enhances matrix degradation through p38 MAPK pathway in osteoarthritis

Fig. 3

a Stem-loop RT-qPCR result of miR-23b-3p (left panel) and protein levels of HS6ST2 and MMP13 (right panel) were assayed in SW1353 cells under stimulation by 10 ng/ml TNF-α for 24 h. b, c Western blotting result of HS6ST2 and MMP13 protein in SW1353 cells transfected with 10 nM mimic miR-23b-3p (b) or 50 nM anti-miR-23b-3p sequence (c) and stimulated by 10 ng/ml TNF-α for 24 h. d, e Toluidine blue staining results of SW1353 cells transfected with 10 nM mimic miR-23b-3p (d) or 50 nM anti-miR-23b-3p sequence (e) and stimulated by 10 ng/ml TNF-α for 24 h. Scale bar, 100 μm. Lower panel, statistical analysis of average optical density of matrix staining of toluidine blue. Asterisk (*): compared with mimic NC or anti-NC group. U6 snRNA and GAPDH were used as internal controls in RT-qPCR for miRNA and western blotting detection, respectively. Bars represent standard error of the mean (SEM) from three independent experiments. One representative result and quantitative data from three independent western blotting and toluidine blue staining. Mann–Whitney U test was used to identify statistical differences between two groups. Asterisk (*) or hash (#) stands for P value <0.05

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