Fig. 4: RA-V blocks the interaction between TAK1 and TAB2.
From: Natural cyclopeptide RA-V inhibits the NF-κB signaling pathway by targeting TAK1
a RA-V blocked the exogenous TAK1–TAB2 interaction. HEK293T cells were transfected with Flag-TAK1 and HA-TAB2 for 24 h and then incubated with various concentrations of RA-V for 6 h. The cell lysates were immunoprecipitated with HA antibody and then immunoblotted with the indicated antibodies. b, c RA-V does not disrupt TAK1–TAB1 or TAK1–TRAF6 interactions. HEK293T cells were transfected with Flag-TAK1 and HA-TRAF6 or HA-TAB1 for 24 h and then incubated with RA-V (800 nM) for 6 h. The cell lysates were immunoprecipitated with HA antibody, and immunoblots were performed with the indicated antibodies. d RA-V blocked the endogenous TAK1–TAB2 interaction. HEK293T (left) or RAW264.7 (right) cells were incubated with 800 nM RA-V for 6 h and stimulated with 10 ng/mL TNF-α for 2 h or 1 μg/mL LPS for 3 h, respectively. The cell lysates were immunoprecipitated with TAK1 antibody or control IgG and then immunoblotted with the indicated antibodies. Each experiment was repeated at least three times
