Fig. 5: Metastasis of OS was inhibited by CXCR1 knockdown in Saos2-lung-M cells in vivo. | Cell Death & Disease

Fig. 5: Metastasis of OS was inhibited by CXCR1 knockdown in Saos2-lung-M cells in vivo.

From: CXCR1/Akt signaling activation induced by mesenchymal stem cell-derived IL-8 promotes osteosarcoma cell anoikis resistance and pulmonary metastasis

Fig. 5

a Cultured Saos2-lung-M cells with CXCR1 interference; GFP was used to label positive cells. b Confirmation of CXCR1 knockdown at the mRNA level. c Confirmation of CXCR1 knockdown at the protein level, and the phosphorylation of Akt. d Real-time monitoring of CXCR1 knockdown and scrambled control Saos2-lung-M cells or RFP-labeled MSCs in vivo using the IVIS 200 system. e Quantification of luciferase intensity in tumors in situ. f Quantification of luciferase intensity in metastasized tumors. g Survival of tumor-bearing mice containing CXCR1-knockdown and scrambled control Saos2-lung-M cells. h Comparison of lung metastasis between CXCR1 knockdown and scrambled control Saos2-lung-M cells. i, j Tumor volume was measured and calculated at week 18 in CXCR1-knockdown and scrambled control Saos2-lung-MSC-bearing mice. Data are presented as mean ± S.D. *p < 0.05, **p < 0.01, and ***p < 0.001. All in vitro data were obtained from at least three independent experiments. Each group contained 10 animals

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