Fig. 3: Quantitative imaging flow-cytometric detection of CAD modalities induced by paclitaxel.

The cell death profiling was performed after co-cultures of untreated (red) CMTMR-labeled HCT116^WT, HCT116^+/+, or MCF7 cells with untreated (green) CMFDA-labeled cells or with paclitaxel (PCT)-treated (green) CMFDA-labeled HCT116^WT, HCT116^+/+, or MCF7 cells. Co-cultures have been performed during 12 h (g–i), 24 h (a–c, j–o) or 48 h (d–f) with the indicated concentrations of PCT in the presence or absence of death effector inhibitors. Then cells were sequentially labeled for the simultaneous detection of type I, II, or III cell death as previously described. Means ± SEM are indicated (n = 3). For a–f, asterisk (*) is used for the comparison of “HCT116^+/++PCT-treated HCT116^+/+” with “HCT116^+/++0 nM HCT116^+/+” for AV⁺D7⁻, ampersand (&) is used for the comparison of “HCT116^+/++PCT-treated HCT116^+/+” with “HCT116^+/++0 nM HCT116^+/+” for D7⁺. For g–l, asterisk (*) is used for the comparison of “MCF7+PCT-treated MCF7” cells with “MCF7+0 nM MCF7” for AV⁺D7⁻, and ampersand (&) is used for the comparison of “MCF7+PCT-treated MCF7” with “MCF7+0 nM MCF7” for D7⁺. For m–o, asterisk (*) is used for the comparison of “HCT116^WT+PCT-treated control (Co.) HCT116^WT” with “HCT116^WT+control (Co.) HCT116^WT” for AV⁺D7⁻, ampersand (&) for the comparison of “HCT116^WT+PCT-treated control (Co.) HCT116^WT” cells with “HCT116^WT+control (Co.) HCT116^WT” cells for D7⁺, hash (#) for the comparison of inhibitor-treated cells with respective control cells for AV⁺D7⁻, and dollar symbol ($) for the comparison of inhibitor-treated cells with respective control cells for D7⁺. *^{, #, &}p < 0.05; **^{, ##, $$, &&}p < 0.01; ***^{, ###, &&&}p < 0.001; and ****^{, &&&&}p < 0.0001