Fig. 1: Increased expression of miR-214 in activated HSCs. | Cell Death & Disease

Fig. 1: Increased expression of miR-214 in activated HSCs.

From: MicroRNA-214 promotes hepatic stellate cell activation and liver fibrosis by suppressing Sufu expression

Fig. 1

a Morphology of primary rat HSCs at different time points in vitro culture at ×40 magnification. b Expression of profibrotic mRNAs including α-SMA, COL1α1, and FN and c protein level of α-SMA and FN was upregulated following HSC activation. d Microarray analysis for miRNAs expression was performed using total RNAs extracted from quiescent HSCs (D1) and activated HSCs (D12), n = 3 per group. Heat map representing two-way hierarchical clustering of differentially expressed miRNAs. e Differential expression of miR-214 in primary rat HSCs was validated using RT-qPCR. Error bars denote standard error of the mean. RT-qPCR was performed in duplicate and repeated three times. Values represent the means ± standard deviation (SD), *P < 0.05 and **P < 0.01

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