Fig. 3: miR-214 regulates fibrotic gene expression and promotes HSC cell proliferation. | Cell Death & Disease

Fig. 3: miR-214 regulates fibrotic gene expression and promotes HSC cell proliferation.

From: MicroRNA-214 promotes hepatic stellate cell activation and liver fibrosis by suppressing Sufu expression

Fig. 3

a HSCs were transfected with either antagomiR-214 or NC-miR for 48 h, and the expression of miR-214 was detected by RT-qPCR and b protein levels of FN and α-SMA were examined using Western blotting. c HSCs were transfected with miR-214 mimics or NC-miR for 48 h, and the expression of miR-214 was detected by RT-qPCR and d the protein levels of FN and α-SMA were detected by Western blotting. e Proliferation of HSCs after transfection with antagomiR-214 or NC-miR was assessed using the MTT and f colony formation assays. g Proliferation of HSCs after transfection with miR-214 mimics or NC-miR was assessed using the MTT assay. Relative expression levels are shown as the means ± s.e.m obtained from triplicate experiments (unpaired two-sample Student’s t test, *P < 0.05 and **P < 0.01)

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