Fig. 4: Sufu is a direct target of miR-214. | Cell Death & Disease

Fig. 4: Sufu is a direct target of miR-214.

From: MicroRNA-214 promotes hepatic stellate cell activation and liver fibrosis by suppressing Sufu expression

Fig. 4

a, b Inverse correlation between miR-214 and Sufu expression in (a) rat primary HSCs and b human LX2 cells transfected with miR-214 mimics or antagomiR-214. c, d RT-qPCR analyses of Sufu mRNA levels after transfection of miR-214 mimics or antagomiR in HSCs (c) and in LX2 cells (d). e Potential miR-214 binding sites were predicted in the 3′-UTR of rat or mouse Sufu mRNAs. Blue shading indicates the seed sequence of miR-214. f, g Dual-luciferase reporter assay was performed to verify the binding between (f) miR-214 and rat Sufu mRNA or (g) miR-214 and mouse Sufu mRNA. HEK 293T cells were co-transfected with vectors containing either the wild-type (wt) or mutant (mut) target sites of Sufu and either miR-214 mimics or negative control (NC). Relative luciferase activities (RLUs) were shown as the means ± S.E.M. obtained from triplicate experiments (two-sample Student’s t test, *P < 0.05 and **P < 0.01). n.s. nonsignificant

Back to article page