Fig. 5: miR-214-mediated fibrogenesis depends on Sufu expression. | Cell Death & Disease

Fig. 5: miR-214-mediated fibrogenesis depends on Sufu expression.

From: MicroRNA-214 promotes hepatic stellate cell activation and liver fibrosis by suppressing Sufu expression

Fig. 5

a Overexpression of Sufu by lentivirus vector in HSCs and SUFU mRNA expression was measured by RT-qPCR and b the protein level of SUFU and FN was measured by Western blotting. c Overexpression of Sufu by lentivirus vector in LX2 and SUFU mRNA expression was measured by RT-qPCR and d the protein level of SUFU, FN, and α-SMA were measured by western blotting. e Knockdown of Sufu expression by siRNAs in HSCs and f the protein expression of SUFU and FN was measured by Western blotting. g Rat primary HSCs were treated with GANT-61 for 24 h and the fold changes in the profibrotic markers FN and α-SMA was detected by (h) RT-qPCR or (i) Western blotting. j The mRNA expression of SUFU in patients with cirrhosis or healthy control was measured by RT-qPCR. All results of relative expression values are shown as mean ± s.e.m. obtained from triplicate experiments (unpaired two-sample Student’s t test, *P < 0.05 and **P < 0.01)

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