Fig. 6: Twist1 affects liver fibrogenesis by regulating miR-214. | Cell Death & Disease

Fig. 6: Twist1 affects liver fibrogenesis by regulating miR-214.

From: MicroRNA-214 promotes hepatic stellate cell activation and liver fibrosis by suppressing Sufu expression

Fig. 6

a, b Twist1 overexpressing by lentiviral vectors containing GFP, fluorescence images for GFP expression (a) in HSCs, and (b) LX2 cells at ×100 magnification. c Twist1 overexpression upregulated miR-214 expression and downregulated SUFU expression, and (d) the protein levels of profibrotic markers FN and α-SMA was upregulated by Twist1 in HSCs. e Twist1 overexpression upregulated miR-214 expression and downregulated SUFU expression, and f the protein level of profibrotic markers FN was upregulated by Twist1 in LX2. g The expression of Twist1 in cirrhosis samples or healthy controls was measured by RT-qPCR. h Schematic representation of luciferase reporter constructs harboring wild-type or mutated proximal miR-214 promoter region and i relative luciferase activity (RLU) was measured. Relative expression levels are shown as the means ± s.e.m. obtained from triplicate experiments (unpaired two-sample Student’s t test, *P < 0.05). n.s. nonsignificant

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