Fig. 4: miR-129 inhibited IGF-1 secretion from SCs. | Cell Death & Disease

Fig. 4: miR-129 inhibited IGF-1 secretion from SCs.

From: miR-129 controls axonal regeneration via regulating insulin-like growth factor-1 in peripheral nerve injury

Fig. 4

a Co-localization of IGF-1 and S100 in SCs. The mRNA (b) and protein (c) expression of IGF-1 as well as the IGF-1 secretion in primary SCs transfected with IGF siRNA (si IGF-1) was significantly decreased as compared to that in SCs transfected with siRNA control (si Con). The expression change of IGF-1 at mRNA (d) and protein levels (e) in SCs transfected with miR-129 mimic (miR-129) and miR-129 inhibitor (anti-miR-129). β-actin was used as an internal control. f IGF-1 secretion was reduced from SCs transfected with miR-129 mimic (miR-129) but was increased from SCs transfected with miR-129 inhibitor (anti-miR-129). g The expression of IGF-1 was not significantly affected by mutant miR-129 mimic both on mRNA level and protein level (h). *p < 0.05 and **p < 0.01 versus respective controls. NC (mimic control), anti-NC (inhibitor control), Mutant miR-129 (mutated miR-129 mimic)

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