Fig. 1: Increased expression of full-length p53 and Δ133p53α in CR cells. | Cell Death & Disease

Fig. 1: Increased expression of full-length p53 and Δ133p53α in CR cells.

From: Δ133p53α, a natural p53 isoform, contributes to conditional reprogramming and long-term proliferation of primary epithelial cells

Fig. 1

a Immunoblot analysis of full-length p53 and Δ133p53α proteins in HPECs (prostate-1) cultured in keratinocyte growth medium (KGM) or CR culture (CRC) for 1–7 passages after four passages in KGM (+P1 through +P7). GAPDH was used as a loading control for quantification. Normalized densitometric values for expression levels are indicated below each lane relative to +P1 KGM cells (defined as 1.0). b Immunoblot analysis of full-length p53 and Δ133p53α proteins in HFKs (foreskin-1) cultured in KGM or CRC for 2–5 passages after four passages in KGM (+P2 through +P5), using GAPDH as the loading control. Normalized densitometric values for expression levels are indicated below each lane relative to +P2 KGM cells (defined as 1.0). Immunoblot analysis of Δ133p53α proteins in HMEC and HEC cells cultured in KGM or CRC are shown in Supplementary Information Fig. S2. Uncropped blot images are shown in Supplementary Information Figs. S20–23

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