Fig. 7: Δ133p53α immortalizes primary epithelial cells in cooperation with Y-27632. | Cell Death & Disease

Fig. 7: Δ133p53α immortalizes primary epithelial cells in cooperation with Y-27632.

From: Δ133p53α, a natural p53 isoform, contributes to conditional reprogramming and long-term proliferation of primary epithelial cells

Fig. 7

a hTERT-overexpressing HFKs (HFK/hTERT-OE) were cultured in KGM with or without Y-27632 beginning three passages after selection. Cumulative population doubling levels (PDLs) were plotted vs. time (days). The HFKs were generated from foreskin-1. b Δ133p53α-overexpressing HPECs (originated from prostate-1 and prostate-2), and c Δ133p53α-overexpressing HFKs (originated from foreskin-1 and foreskin-2) were cultured in KGM with or without Y-27632 beginning 4–5 passages after selection as indicated. Cumulative PDLs were plotted vs. time (days)

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