Fig. 1: Experimental strategy and RISC-associated miRNAs and mRNAs.

a Experimental plan outline. b, c Volcano plot of differentially modulated miRNAs and mRNAs, revealed by RISC-associated RNA-sequencing. miRNAs (b) and mRNAs (c), differentially modulated in RISC complex of DM1 patients compared to CTRs. Red dots represent miRNAs and mRNAs displaying statistically significant differences (FDR < 0.1; DM1, n = 3; CTR, n = 3). miRNAs with mRNA targets differentially modulated in the RISC, and validated mRNAs are identified by name in b and c, respectively