Fig. 2: Modulation of miRNAs and mRNAs in skeletal muscle tissue. | Cell Death & Disease

Fig. 2: Modulation of miRNAs and mRNAs in skeletal muscle tissue.

From: High-throughput analysis of the RNA-induced silencing complex in myotonic dystrophy type 1 patients identifies the dysregulation of miR-29c and its target ASB2

Fig. 2

a, b Comparison between RNA-Seq results (DM1, n = 3; CTR, n = 3) and qPCR validation (DM1, n ≥ 5; CTR ≥ 5) in RISC-IP samples of DM1 compared to CTR subjects. Data are represented in Log2 scale, (−ΔΔCt), referred to CTRs. Upregulated and downregulated genes are indicated in red and in green, respectively. a miRNAs; miR-381 was not detectable by qPCR in both input and IP RNAs; b mRNAs; mRNA RNA-Seq of input RNA was not performed. c Dot plots of miR-29c and ASB2 mRNA validation experiments. RISC-IP associated RNAs (IP) and INPUT RNAs obtained from biopsies of DM1 patients vs healthy subjects (CTR) were analyzed by qPCR and normalized to miR-181a and RPL23 mRNA, respectively. Values are indicated as −1*ΔΔCt fold changes (log2FC). Average and error bars are also indicated (CTR n ≥ 5; DM1 n ≥ 5; *P < 0.05; **P < 0.01). White circles: CTR; black circles: DM1

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