Fig. 6: Cisplatin inhibited PPP2CA expression through upregulation of YY1 expression. | Cell Death & Disease

Fig. 6: Cisplatin inhibited PPP2CA expression through upregulation of YY1 expression.

From: Knockdown of Yin Yang 1 enhances anticancer effects of cisplatin through protein phosphatase 2A-mediated T308 dephosphorylation of AKT

Fig. 6

a, b Expressions of YY1 and PPP2CA after treatment with cisplatin. CAL27 cells were exposed to different dosage of cisplatin for 48 h (a); CAL27 cells were exposed to cisplatin (10 μM) and protein was collected at indicated time points (b), total proteins were subjected to Western blot analysis. c, d Knockdown of YY1 rescued cisplatin-induced PPP2CA protein inhibition. CAL27 cells stably infected with YY1 shRNA or scramble shRNA lentivirus and cells exposed to different dosage of cisplatin (c). Proteins were extracted from xenograft tumors (d) and total proteins were subjected to Western blot analysis. a–d The target bands were exposed, and densitometry was performed as fold change ratio from the mean of three independent experiments, one-way ANOVA: *P < 0.05 vs. control group

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