Fig. 3: Knockout of miR-34a could promote the dedifferentiation of U-2 OS cells.
From: miR-34a exerts as a key regulator in the dedifferentiation of osteosarcoma via PAI-1–Sox2 axis
a Morphological changes in miR-34a-depleted U-2 OS compared to WT U-2 OS. b, c qRT-PCR (b) and western blotting (c) analysis of Sox2 expression in WT U-2 OS and miR-34a-depleted U-2 OS. d, e Soft agar colony formation assay to investigate the self-renewal ability of WT U-2 OS cells and miR-34a-depleted U-2 OS. f Adipogenic differentiation of WT U-2 OS cells and miR-34a-depleted U-2 OS. g qRT-PCR analysis of adipogenic markers PPARγ, aP2, GLUT4 and LPL mRNA expression level in WT U-2 OS cells and miR-34a-depleted U-2 OS after adipogenic differentiation. Scale bar = 100 μm. *P < 0.05, **P < 0.01
