Fig. 2: JQ1 induces apoptosis, blocks cell growth and enhances the radiosensitivity of NPC cells. | Cell Death & Disease

Fig. 2: JQ1 induces apoptosis, blocks cell growth and enhances the radiosensitivity of NPC cells.

From: BET bromodomain inhibitor JQ1 preferentially suppresses EBV-positive nasopharyngeal carcinoma cells partially through repressing c-Myc

Fig. 2

a Annexin V and propidium iodide (PI) staining of CNE2-EBV−/+ and TWO3-EBV−/+ cells treated with DMSO, 2.5 μM JQ1 or 10 μM JQ1 for 48 h. Experiments were repeated 3 times and representative images are shown. b Quantification of Annexin V positive cells shown in a. Data are presented as mean ± SD of 3 independent experiments. c Colony formation assays of CNE2-EBV−/+ and TWO3-EBV−/+ cell lines. Cells were cultured in the presence of DMSO, 0.1 μM JQ1 or 1 μM JQ1 for 10–14 days followed by crystal violet staining. Representative photographs are shown. d Number of colonies for CNE2-EBV−/+ and TWO3-EBV−/+ cells treated with DMSO or 0.1 μM JQ1. e Invasion assays of CNE2-EBV−/+ and TWO3-EBV−/+ cell lines with or without JQ1 treatment. JQ1 was used at 50 nM. Quantification of invasive cells shown below. f Clonogenic cell surviving curves of CNE2 and CNE2-EBV+ cells that were pretreated with JQ1 (500 nM) or DMSO for 24 h and then exposed to 2, 4, 6 or 8 Gy of X-ray irradiation. 48 h after irradiation, media were replaced by fresh drug-free media. Colony-forming efficiency was determined 10–14 days later. The data are reported as mean ± SD of 3 independent repeats. *P < 0.05; #P < 0.01

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